The City of Iola has a bicycle route network, designated by sharrows painted on city streets. The route connects the historic downtown square to the Prairie Spirit Trail, the Southwind Rail Trail, the Missouri Pacific Recreational Trail, and the Lehigh Portland Trails, via the King of Trails Bridge.

The route uses Washington Avenue as the main north-south arterial, with a spur to the Prairie Spirit Trail on West Street, and a spur to the Southwind Rail Trail on Vine and Park Streets. The route totals approximately 2 miles.